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KMID : 0380020090240040341
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 4 p.341 ~ p.346
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Functional Expression of Candida antarctica Lipase A in Pichia a pastoris and Escherichia coli
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Park Hye-Jung
Kim Yong-Hwan
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Abstract
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Candida Antarctica lipase A (CalA) has been used because of its suitability in industrial applications. CalA has unique features capable to accept tertiary and sterically hindered alcohols among many hydrolases. CalA gene was cloned and constructed in expression vector such as pColdIII/CalA and /CalA. The gene encoding pColdIII/CalA was functionally expressed in the cytoplasm of Escherichia coli B (DE3) cells. The plasmid /CalA linearized by BstX I was integrated into 5’AOX1 region of the chromosomal DNA and was functionally expressed in the methyl atrophic yeast Pichia pastoris. Expressed CalA in P. pastoris (0.7 Unit/mL) showed 35 times higher activity than that in E. coli expression system (0.02 Unit/mL).
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KEYWORD
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Candida antarctica lipase A, P. pastoris, E. coli, AOX 1
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